Apẹrẹ Ọjọgbọn Didara Didara Proteinase K CAS No.. 39450-01-6 pẹlu Owo to dara julọ

Nigbagbogbo a n ṣe jijẹ agbara oṣiṣẹ ojulowo ni idaniloju pe a yoo fun ọ ni anfani ti o dara julọ pẹlu idiyele tita to dara julọ fun Didara Didara Oniru ỌjọgbọnProteinase K CAS No.. 39450-01-6pẹlu Ti o dara ju Price, Fun afikun alaye ati awọn mon, jọwọ sọrọ si wa ni yarayara bi o ti ṣee!
Nigbagbogbo a n ṣe jijẹ iṣẹ ṣiṣe ojulowo ni idaniloju pe a yoo fun ọ ni anfani ti o dara julọ pẹlu idiyele tita to dara julọ funChina Tritirachium Album Limber ati Molecular Biology, Iṣakoso didara ti o muna ni a ṣe ni ọna asopọ kọọkan ti gbogbo ilana iṣelọpọ.Da lori awọn solusan didara ti o ga ati pipe ṣaaju-tita / iṣẹ lẹhin-tita ni imọran wa, diẹ ninu awọn alabara ti ṣe ifowosowopo pẹlu wa diẹ sii ju ọdun 5 lọ.

Proteinase Kjẹ protease serine ti o jẹ ti idile subtilisin pẹlu iṣẹ ṣiṣe enzymatic ti o munadoko ati pato sobusitireti gbooro, eyiti o fẹfẹ decomposes awọn iwe adehun ester ati awọn peptides ti o wa nitosi C-terminus ti amino acids hydrophobic, amino acids ti o ni imi-ọjọ imi, ati awọn ifunmọ amino acids aromatic, eyiti o jẹ nigbagbogbo lo lati degrade awọn ọlọjẹ lati gbe awọn peptides kukuru.Proteinase Kni ihuwasi ti triad catalytic aṣoju Asp39-His69-Ser224 alailẹgbẹ si awọn proteases serine ati pe o ni awọn aaye abuda Ca2 + meji ni ayika ile-iṣẹ ti nṣiṣe lọwọ lati mu iduroṣinṣin rẹ pọ si ati ṣetọju iṣẹ ṣiṣe enzymatic giga labẹ iwọn awọn ipo pupọ.

1. Jiini okunfa kit.
2. RNA ati DNA isediwon irin ise.
3. Jade awọn ohun elo ti kii ṣe amuaradagba ninu awọn tisọ ati awọn idoti amuaradagba (gẹgẹbi igbaradi ti awọn ajesara DNA ati heparin).
4. Igbaradi ti Chromosomal DNA fun Pulse Electrophoresis.
5. Western blotting.
6. Idagbasoke ati iṣelọpọ ti enzymatic glycated albumin reagents ni aaye ti awọn iwadii aisan in vitro.

1. 30mg / igo 1g / igo tabi 100g / igo
2. 1kgs / bankanje apo

Proteinase K jẹ protease serine iduroṣinṣin pẹlu pato sobusitireti gbooro.O degrades ọpọlọpọ awọn ọlọjẹ ni ilu abinibi ipinle ani niwaju detergents.Ko muu ṣiṣẹ nipasẹ awọn ions irin, awọn aṣoju chelating (fun apẹẹrẹ, EDTA), awọn reagents sulfhydryl, tabi nipasẹ awọn inhibitors tripsin tabi chymotrypsin.O jẹ iduroṣinṣin lori iwọn pH jakejado (4-12.5), pẹlu iṣẹ ṣiṣe to dara julọ ni pH 6.5-9.5.Iṣẹ ṣiṣe le jẹ jijẹ nipasẹ afikun ti awọn aṣoju denaturing (SDS ati Urea).Iyara denaturation ti enzymu waye ni iwọn otutu ti o ga ju 70°C.Aifọwọyi ti henensiamu waye ni ilọsiwaju ni pH ipilẹ.Sibẹsibẹ, Proteinase K ko ṣiṣẹ patapata nipasẹ adaṣe adaṣe.Diẹ ninu awọn ajẹkù henensiamu tẹsiwaju lati ṣetọju iṣẹ ṣiṣe proteolytic pipe wọn, paapaa lẹhin adaṣe adaṣe lọpọlọpọ.Amuaradagba K ti wa ni lilo nigbagbogbo ni awọn ohun elo isedale molikula lati da awọn ọlọjẹ ti aifẹ, gẹgẹbi awọn iparun ni DNA tabi awọn igbaradi RNA lati awọn microorganisms, awọn sẹẹli gbin, ati awọn ohun ọgbin.Enzymu jẹ igbagbogbo lo ni 50-200 ug/ml ni awọn igbaradi acid nucleic ni pH 7.5-8.0 ati 37 ~ 55oC.Awọn akoko ifibọ yatọ lati iṣẹju 30 si wakati 18.

Serine protease iduroṣinṣin ati ifaseyin pupọ;ti a lo fun amuaradagba ati ipinya acid nucleic.Proteinase K ni a lo ninu isọdimọ ti RNA ati DNA lati awọn tissu tabi awọn laini sẹẹli.

Nigbagbogbo a n ṣe jijẹ agbara oṣiṣẹ ojulowo ni idaniloju pe a yoo fun ọ ni anfani ti o dara julọ pẹlu idiyele tita to dara julọ fun Amuaradagba Didara Didara Didara K.CAS No.. 39450-01-6pẹlu Ti o dara ju Price, Fun afikun alaye ati awọn mon, jọwọ sọrọ si wa ni yarayara bi o ti ṣee!
Oniru ỌjọgbọnChina Tritirachium Album Limber ati Molecular Biology, Iṣakoso didara ti o muna ni a ṣe ni ọna asopọ kọọkan ti gbogbo ilana iṣelọpọ.Da lori awọn solusan didara ti o ga ati pipe ṣaaju-tita / iṣẹ lẹhin-tita ni imọran wa, diẹ ninu awọn alabara ti ṣe ifowosowopo pẹlu wa diẹ sii ju ọdun 5 lọ.